Abstract
Background
Osteoarthritis (OA) is a joint disease of multifactorial etiology, affecting mainly
the knees. We aimed to evaluate the effects of two different doses of gaseous ozone
intra-articularly on the knee cartilage morphology of rats with osteoarthritis (OA).
Methods
The articular lesion was induced by sodium monoiodoacetate (MIA). 40 Wistar rats were
divided into 4 groups: G1 control (without lesion and without treatment), G2 articular
lesion (AL) (only lesion MIA-induced), G3 AL + treatment with 5 μg/mL of ozone intra-articular,
and G4 AL + treatment with 10 μg/mL of ozone intra-articular. The experiment was carried
out for 60 days.
Results
Both doses of ozone intra-articular demonstrated less reduction in joint space (G3
and G4) compared to the G2, formation of osteophytes, but without subchondral sclerosis.
Ozone decreased the volumetric density of the articular lesion (VV(AL)) of tibial.
The treatments recovered VV(AL) of the femur similar to G1. Ozone lower dose (G3)
showed lower tibia and femur macroscopic scores.
Conclusion
Intra-articular gaseous ozone can delay the degeneration of articular cartilage and
can represents an integrative therapy in the OA treatment of knee after 60 days of
treatment. For the first time the role of ozone in articular cartilage degeneration
was evaluated helping to understand this therapy.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to The KneeAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- To what extent is severe osteoarthritis preventable? Occupational and non-occupational risk factors for knee and hip osteoarthritis.Rheumatology. 2020; 59: 3869-3877https://doi.org/10.1093/rheumatology/keaa238
- A randomized controlled trial of a combined self-management and exercise intervention for elderly people with osteoarthritis of the knee: the PLE2NO program.Clin Rehabil. 2018; 32: 223-232https://doi.org/10.1177/0269215517718892
- Role of IL-17 gene polymorphisms in osteoarthritis: A meta-analysis based on observational studies.World J Clin Cases. 2020; 8: 2280-2293https://doi.org/10.12998/wjcc.v8.i11.2280
- Desempenho Muscular, Dor, Rigidez E Funcionalidade De Idosas com Osteoartrite de Joelho.Acta Ortopédica Bras. 2011; 19: 193-197https://doi.org/10.1590/S1413-78522011000400004
- Métodos fisioterapêuticos utilizados na reabilitação do equilíbrio postural em indivíduos com osteoartrite: uma revisão sistemática.ABCS Heal Sci. 2018; 43: 55-60https://doi.org/10.7322/abcshs.v43i1.934
- Estudo comparativo entre a classificação radiólogica e análise macro e microscópica das lesões na osteoartrose do joelho.Rev Bras Ortop. 2011; 46: 155-159https://doi.org/10.1590/S0102-36162011000200007
- Plain radiography and magnetic resonance imaging diagnostics in osteoarthritis: Validated staging and scoring.J Bone Jt Surg - Ser A. 2009; 91: 54-62https://doi.org/10.2106/JBJS.H.01385
Jesus CCL de, Santos FC dos, Oliveira LM, Jesus B de, Monteiro I, Sant’Ana MSSC, et al. Comparison between intra-articular ozone and placebo in the treatment of knee osteoarthritis : A randomized , double-blinded , placebo-controlled study. PLoS One 2017;12:1–16.
- Ozone therapy as an integrating therapeutic in osteoartrosis treatment: a systematic review.Brazilian J Pain. 2018; 1: 171-175https://doi.org/10.5935/2595-0118.20180033
- Efficacy of combined ozone and platelet-rich-plasma treatment versus platelet-rich-plasma treatment alone in early stage knee osteoarthritis.J Back Musculoskelet Rehabil. 2019; 32: 305-311https://doi.org/10.3233/BMR-181301
- Intra-articular ozone modulates inflammation, ameliorates pain and rigidity, improves function and has anabolic effect on knee osteoarthritis: A prospective quasi-experimental before-and-after study, 115 patients.Rev La Soc Esp Del Dolor. 2020; 27: 78-88https://doi.org/10.20986/resed.2020.3775/2019
- Clinical utility of ozone therapy for musculoskeletal disorders.Med Gas Res. 2018; 8: 103-110https://doi.org/10.4103/2045-9912.241075
- Intra-articular ozone therapy efficiently attenuates pain in knee osteoarthritic subjects: A systematic review and meta-analysis.Complement Ther Med. 2019; 42: 240-247https://doi.org/10.1016/j.ctim.2018.11.023
ANDRADE A, PINTO SC, OLIVEIRA RS De. Animais de laboratório: criação e experimentação. Rio de Janeiro: Fiocruz; 2002.
OECD. OECD Guidelines for the testing of chemicals: Repeated Dose 28-day Oral Toxicity Study in Rodents. vol. 34. 2008. 10.1787/20745788.
OECD. OECD Guideline for the testing of chemicals: Repeated dose 90-day oral toxicity in rodents. 2018. 10.1787/9789264070707-en.
- Osteoarthritis model induced by intra-articular monosodium iodoacetate in rats knee.Acta Cir Bras. 2016; 31: 765-773https://doi.org/10.1590/S0102-865020160110000010
- The role of radiography and MRI for eligibility assessment in DMOAD trials of knee OA.Nat Rev Rheumatol. 2018; 14: 372-380https://doi.org/10.1038/s41584-018-0010-z
- Practical Methods for Biological Morphometry.J Microsc. 1979; 121: 131-132
- Monoiodoacetic acid induces arthritis and synovitis in rats in a dose- and time-dependent manner: Proposed model-specific scoring systems.Osteoarthr Cartil. 2016; 24: 1284-1291https://doi.org/10.1016/j.joca.2016.02.005
- Development and characterization of various osteoarthritis models for tissue engineering.PLoS ONE. 2018; 13: e0194288https://doi.org/10.1371/journal.pone.0194288
- A meta-analysis of sex differences prevalence, incidence and severity of osteoarthritis.Osteoarthr Cartil. 2005; 13: 769-781https://doi.org/10.1016/j.joca.2005.04.014
- Prophylactic Ozone Administration Reduces Intestinal Mucosa Injury Induced by Intestinal Ischemia-Reperfusion in the Rat.Mediators Inflamm. 2015; 2015: 1-8https://doi.org/10.1155/2015/792016
- Avaliação radiológica do espaço articular na artrose do quadril: estudo comparativo em decúbito e ortostatismo.Rev Bras Ortop. 2008; 43: 460-464https://doi.org/10.1590/S0102-36162008001000006
- Supracondylar femoral osteotomy and knee joint replacement during the same surgical procedure in a type A haemophiliac patient with knee flexion deformity and ankylosis.Knee. 2017; 24: 477-481https://doi.org/10.1016/j.knee.2016.11.007
Chapman J, Bansal P, Goyal A, Azevedo AM. Splenomegaly. StatPearls [Internet]; 2020.
- Ozone therapy: An overview of pharmacodynamics, current research, and clinical utility.Med Gas Res. 2017; 7: 212-219https://doi.org/10.4103/2045-9912.215752
- The efficacy of ozone therapy in experimental caustic esophageal burn.J Pediatr Surg. 2008; 43: 1679-1684https://doi.org/10.1016/j.jpedsurg.2008.01.064
- Medical ozone therapy as a potential treatment modality for regeneration of damaged articular cartilage in osteoarthritis.Saudi J Biol Sci. 2018; 25: 672-679https://doi.org/10.1016/j.sjbs.2016.02.002
- Potencial regenerativo do tecido cartilaginoso por células-tronco mesenquimais: atualização, limitações e desafios.Rev Bras Ortop. 2017; 52: 2-10https://doi.org/10.1016/j.rbo.2016.02.007
- Mechanotransduction pathways in the regulation of cartilage chondrocyte homoeostasis.J Cell Mol Med. 2020; 24: 5408-5419https://doi.org/10.1111/jcmm.15204
Raeissadat SA, Rayegani SM, Moridnia M, Dehgolan SR. AB1246-HPR Intra articular ozone or hyaluronic acid injection: which one is superior in patients with knee osteoarthritis? a 6-month randomized clinical trial. Heal. Prof. Rheumatol. Abstr., vol. 1, BMJ Publishing Group Ltd and European League Against Rheumatism; 2017, p. 1547.2-1548. 10.1136/annrheumdis-2017-eular.5484.
- Ozone oxidative postconditioning ameliorates joint damage and decreases pro-inflammatory cytokine levels and oxidative stress in PG/PS-induced arthritis in rats.Eur J Pharmacol. 2013; 714: 318-324https://doi.org/10.1016/j.ejphar.2013.07.034
- To What Extent Does Ozone Therapy Need a Real Biochemical Control System? Assessment and Importance of Oxidative Stress.Arch Med Res. 2007; 38: 571-578https://doi.org/10.1016/j.arcmed.2007.03.002
- Is it true that ozone is always toxic? The end of a dogma.Toxicol Appl Pharmacol. 2006; 216: 493-504https://doi.org/10.1016/j.taap.2006.06.009
- Modulation of oxidative stress by ozone therapy in the prevention and treatment of chemotherapy-induced toxicity. Review and prospects.Antioxidants. 2019; 8: 588https://doi.org/10.3390/antiox8120588
- Medical ozone promotes Nrf2 phosphorylation reducing oxidative stress and pro-inflammatory cytokines in multiple sclerosis patients.Eur J Pharmacol. 2017; 811: 148-154https://doi.org/10.1016/j.ejphar.2017.06.017
- Anti-inflammatory Effect of Ozone Therapy in an Experimental Model of Rheumatoid Arthritis.Inflammation. 2020; 43: 985-993https://doi.org/10.1007/s10753-020-01184-2
- Ozone in Medicine: The Low-Dose Ozone Concept-Guidelines and Treatment Strategies.Ozone Sci Eng J Int Ozone Assoc. 2012; 34: 408-424https://doi.org/10.1080/01919512.2012.717847
Article info
Publication history
Published online: March 17, 2022
Accepted:
March 3,
2022
Received in revised form:
November 29,
2021
Received:
March 27,
2021
Footnotes
☆ANOVA, analysis of variance; AP, anteroposterior; AS, articular surfaces; IL, interleukin; MIA, sodium monoiodoacetate; OA, Osteoarthritis; P, profile; TNF-α, tumor necrosis factor alpha; AL, articular lesion; VV, volumetric density; VV(AS), VV of the articular surface; VV(AL), VV of the articular lesion.
Identification
Copyright
© 2022 Elsevier B.V. All rights reserved.
